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Journal of the American Animal Hospital Association 42:326-335 (2006)
© 2006 American Animal Hospital Association

Pearls of Veterinary Practice


Molecular Biology for the Clinician: Understanding Current Methods

Heather L. Chandler, BS and Carmen M.H. Colitz, DVM, PhD, Diplomate ACVO

From the Departments of Veterinary Clinical Sciences (Colitz) and Veterinary Bioscience (Chandler), College of Veterinary Medicine, Ohio State University, Columbus, Ohio 43210.

The basics of molecular biology involve the replication of deoxyribonucleic acid and its transcription and translation into proteins. Biochemical assays such as the Southern blot analysis, polymerase chain reaction (PCR), Northern blot analysis, reverse-transcriptase PCR, microarray technology, Western blot analysis, immunohistochemistry, enzyme-linked immunosorbent assay, and flow cytometry utilize various aspects of molecular biology. To understand these assays requires some basic understanding of the principles of molecular biology. This paper provides basic information on the methodology and techniques used in these assays.






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Copyright © 2006 by the American Animal Hospital Association.